# Microorganisms

Microorganisms Definition: The organisms which are too small to be seen by the naked eyes & can be seen only through a microscope are called microorganisms.

## Occurrence of Microbes

Microorganisms are present everywhere in the air, soil, water, foodstuffs, sewage, waste as well as inside of any living bodies. The maximum number of microbes are found in the natural substrates which are rich in organic food and have a suitable temperature and moisture for their growth and multiplication. An important fact about these microorganisms is that they are strong. They can survive extreme conditions of temperature and dryness by forming a cyst, which is a hard outer coat around their bodies.

## Major Groups of Microorganisms

Microorganisms are present in the air, water, food, and even inside our bodies. While a majority of the microbes are harmless, some of them have the capability of causing dangerous diseases.

Microbes are mainly classified into five major groups :

1. Bacteria
2. Algae
3. Fungi
4. Protozoa
5. Viruses

### Bacteria

Bacteria (singular: bacterium) are single-celled organisms that vary in shape and size. Some bacteria are round or oval (coccus), others are rod-shaped (bacillus) or spiral. Some bacteria are autotrophic as they contain chlorophyll, but most of them are heterotrophic, showing the saprophytic or parasitic mode of nutrition.

### Algae

Autotrophic organisms having undifferentiated plant bodies are called algae (singular: alga). Algae occur abundantly, like grass in aquatic habitats, and are also called ‘grass of water’.

### Fungi

• Fungi (singular: fungus) are a group of plant-like organisms exhibiting heterophic (saprophytic or parasitic) nutrition. They are commonly found on bread, leather, cotton, paper, etc. Fungi grow vigorously in damp, warm, dark places, Yeast, moulds and mushrooms are forms of fungi. Yeast is commonly used for baking bread, cakes, etc. The yeast ferments sugar present in the wheat and converts it to carbon dioxide and alcohol.

### Protozoa

• Unicellular organisms that exhibit animal-like characteristics are called protozoa (singular: protozoan). The word protozoan literally means ‘the first animal’. Most protozoa are heterotrophic.

### Viruses

• A virus is a microorganism that exhibits characteristics of living as well as a non-living thing. Viruses exhibit a variety of shapes, having a simple structure. Reproducing only inside a living organism, viruses can cause many diseases.

## Use of Bacteria

### In Medicine :

Antibiotics: Many well-known antibiotics such as streptomycin, aureomycin, and chloromycin are obtained from bacteria.

### In Agriculture :

The decay of Organic Substances: Bacteria act on dead bodies of animals and plants and convert various complex organic compounds into simple inorganic substances. Thus, bacteria help in the recycling matter.

Nitrification: The protein confined in dead bodies of animals and plants is converted into ammonia by ammonifying bacteria. This process is known as ammonification. This ammonia is converted into nitrites by bacteria of the Nitrosomonas group.

Nitrogen Fixation: Some of the soil bacteria like Azotobacter, Clostridium, and symbiotic bacteria (Rhizobium found in root nodules of leguminous plants) are capable of fixing free atmospheric nitrogen into nitrates. These nitrates are used by green plants to produce proteins.

### In Industry :

Lactobacillus bacteria help in the formation of milk products such as curd and cheese.

Some bacteria are also used in the preparation of industrial products such as lactic acid, acetone, alcohol etc.

## Use of Fungi

### In Medicine :

• Many well-known antibiotics are obtained from fungi. Penicillin is obtained from the fungus Penicillium notatum. Griseofulvin is extracted from the fungus Griseofulvum.

### In Industry :

Brewery and Bakery: The wine (brewery) and bread (bakery) industries are the two most important industries which make use of the fungus yeast. Both these industries are based on the fermentation activity of yeast.

Glucose Alcohol + Carbon dioxide

Vitamins: Yeast is an important source of vitamin B-complex.

Preparation of Organic Acids: Fermentation properties of several fungi are utilized for the manufacture of oxalic acid, citric acid, etc.

### In Agriculture :

• Fungi decompose the dead bodies of plants, animals, and their waste products.

### As Food

Some species of fungi are edible. They are cooked like vegetables or used in pulao or prepared as soups. Some common edible fungi are Morchella, Agaricus, Ramaria, Clavaria, and Pleurotus.

Yeast is also used in the preparation of idli and dosa from a mixture of powdered rice and dal.

## Use of Algae

Algae play an important role in medicine, agriculture, industry and as food.

### In Medicine :

• The green algae chlorella yield an important antibiotic called chlorellin.

### In Industry :

Algal Products: Agar and algin are obtained from red and brown algae respectively. These are used in the preparation of medicines, food, and cosmetics.

Source of Iodine (I), Sodium (Na), and Potassium (K): The brown algae are the most important sources of sodium, potassium and iodine. Iodine is obtained from an alga called kelp.

### In Agriculture:

As Manure: Many of the seaweeds are directly added to agricultural fields as manure because they are rich in nitrogen and potassium.

Nitrogen Fixation: Some blue-green algae like Nostoc and Anabaena are important in the fixation of atmospheric nitrogen in soils.

Food: The red alga Porphyra is used directly as food in China and Japan.

Fodder: Some seaweeds such as Laminaria are used as fodder for sheep and cattle.

## Use of Protozoa

Some free-living protozoa form an important link in an aquatic food chain. They feed on algae and are, in turn, eaten by bigger animals.

Some forms of protozoa help in the final degradation of waste and sewage.

Protozoa are used as research material for various biological activities.

## Harmful Micro Organism

The disease-causing microorganisms are called pathogens. It is necessary to protect ourselves from the different diseases caused by microorganisms or germs. The commonest mode of entry for these germs is the food we eat and the water we drink. Some harmful microorganisms and the diseases caused are given in Table :

 Table Harmful Microorganisms Classification Species/Name Diseases caused Bacteria Salmonella, Clostridium Food poisoning Mycobacterium, etc. Tuberculosis, whooping cough, cholera, typhoid Algae Cephaleuros Red rust of tea and coffee plants Fungi Moulds, mildews Spoilage of food, rust of wheat, the blight of potatoes Ringworm, etc. Fungal infection, athlete’s foot Protozoa E. histolytica Amoebiasis, dysentery Plasmodium Malaria Viruses Measles virus Measles Poliovirus Poliomyelitis HIV AIDS

Disease carrying germs are carried by other organisms. For e.g. The pathogen for malaria is carried by a mosquito (the female Anopheles, mosquito ) and enters the body when the mosquito bites. Here, the mosquito acts as a carried while the human is the host. Since mosquitoes are capable of carrying and spreading many diseases like malaria, dengue, etc. It is very important to control the reproduction or breeding of mosquitoes. Since mosquitoes breed on water, we must ensure that there is no standing rainwater or water in coolers, etc. near our homes.

## Method of Food Preservation

What do you do with left-over food after dinner? It is kept in the fridge. If left outside, the food can get spoiled and can cause food poisoning if eaten. We have seen how dangerous microorganisms can be. That is why it is very important for us to be careful about food quality and preservation. Different methods used to prevent spoilage of food, by limiting the growth of microbes are :

### Refrigeration :

• Food is kept at a low temperature to reduce the rate of reproduction of the microorganisms and prevent food spoilage.

### Dehydration or drying :

• Food items are dried in the sun or by spraying or freezing. This process is commonly used for preserving fruits, although some nutrients are lost by drying.

### Addition of salt or sugar :

• Microbes need water for growth. By adding salt or sugar, water is made unavailable, thereby controlling microbial growth. Pickles and jams are preserved food items (fruits and vegetables) and can be kept for many months.

### Freezing :

• Frozen food is very popular nowadays as it has a greater shelf life, without much loss of nutrients. Reduction in temperature and unavailability of water help to limit microbes.

### Smoking :

Meat products are preserved by the process of smoking which uses drying techniques as well as some substances from the smoke.

### Pasteurization and Homogenization :

• Pasteurization is a process by which microorganisms are destroyed by high temperature and sudden cooling. Milk is heated to around 70ºC and then suddenly cooled. It is commonly used for milk since other food items can lose heat-sensitive nutrients.
• Homogenization is the process of passing a substance (usually milk) under high pressure through thin tubes, which reduces the tendency of creaming of fat.

### Canning :

• Canned products are first subjected to high heat processing, then sealed in cans. While canning
increases shelf life.

### Sterilization :

• Ionizing radiations are used to destroy microorganisms without affecting heat-sensitive nutrients. This process is commonly used for the preservation of mushrooms, strawberries, potatoes, etc.

## Nitrogen Fixation

The process of converting atmospheric nitrogen into compounds of nitrogen, mainly nitrates, which can be easily used by plants is called nitrogen fixation. It can be fixed in two ways.

1. Natural fixation or Atmospheric fixation
2. Artificial fixation or Industrial fixation.

### Natural Fixation of Nitrogen :

• In nature nitrogen of the atmosphere can be fixed by three methods.

#### Fixation of Nitrogen by Bacteria :

• The root nodules of certain leguminous plants contain nitrogen-fixing bacteria called Rhizobium. The bacteria can directly fix nitrogen gas to nitrogen compounds which can utilized by the plants.

#### Fixation of Nitrogen by Blue – Green Alge :

• Blue-green algae like Nostoc and Anabaena can also help in nitrogen fixation. The fixation of nitrogen by bacteria and algae is called the biological fixation of nitrogen.

#### Fixation of Nitrogen by Lighting :

• During lighting in the sky, when a high temperature is produced, the nitrogen gas of the atmosphere reacts with oxygen to form nitrogen oxide. This nitrogen oxide dissolves in rainwater to form a very dilute solution of nitric acid. The nitric acid thus formed reacts with alkalis present in the soil to form nitrates which are then absorbed by the plants.

### Artificial fixation of Nitrogen :

• In this method, the atmospheric nitrogen is made to combine with hydrogen gas to form ammonia (Haber’s process). This ammonia can be oxidized to make nitrates or react with acid to form ammonium salts. The nitrates as well as ammonium salts contain fixed nitrogen and are used as fertilizers.

### Nitrogen Cycle :

• The circulation of nitrogen through the living and non-living components of the biosphere (air, soil, water, plants, and animals) is called the nitrogen cycle. The nitrogen cycle maintains the percentage of nitrogen in the atmosphere more or less at a constant.

### Steps Involved in Nitrogen Cycle :

• The atmospheric nitrogen is fixed into nitrogen compounds like nitrates by Rhizobium bacteria, blue-green algae, lightning, or industrial method.
• The plants absorb nitrate compounds from the soil
and water and convert them into plant proteins.
• The plants are eaten up by animals and thus plant
proteins are used for making animal proteins.
• When the plants and animals die, bacteria and fungi present in the soil decompose the  proteins of dead plants and animal into ammonia. This process is called ammonification.
• Ammonia thus formed is converted first into nitrites and then into nitrates by the action of Nitrosamines and Nitrobacteria respectively. The process is called nitrification.
• The soil contains denitrifying bacteria called Pseudomonas which convert nitrate form of nitrogen into free nitrogen which goes back into the atmosphere. The process is called denitrification.

### What is Microorganisms?

The organisms which are too small to be seen by the naked eyes & can be seen only through a microscope are called microorganisms.

### How many Types of Microorganisms?

Microbes are mainly classified into five major groups:

1. Bacteria
2. Algae
3. Fungi
4. Protozoa
5. Viruses

### What is Nitrogen Fixation?

The process of converting atmospheric nitrogen into compounds of nitrogen, mainly nitrates, which can be easily used by plants is called nitrogen fixation.